Journal: Plant Methods

Article Title: A high-density Diversity Arrays Technology (DArT) microarray for genome-wide genotyping in Eucalyptus

doi: 10.1186/1746-4811-6-16

Figure Lengend Snippet: Flowchart of the development of the DArT genotyping microarray for Eucalyptus .

Article Snippet: Arrays were spotted using a MicrogridII arrayer (Biorobotics) on poly-L-lysine coated glass microarray slides (Erie Scientific).

Techniques: Microarray

Journal: Plant Methods

Article Title: A high-density Diversity Arrays Technology (DArT) microarray for genome-wide genotyping in Eucalyptus

doi: 10.1186/1746-4811-6-16

Figure Lengend Snippet: Eucalyptu s species and number of individuals of each species (total of 284) used as targets to screen the prototype DArT microarray for polymorphic markers

Article Snippet: Arrays were spotted using a MicrogridII arrayer (Biorobotics) on poly-L-lysine coated glass microarray slides (Erie Scientific).

Techniques: Microarray

Journal: Plant Methods

Article Title: A high-density Diversity Arrays Technology (DArT) microarray for genome-wide genotyping in Eucalyptus

doi: 10.1186/1746-4811-6-16

Figure Lengend Snippet: Summary of results of the Eucalyptus DArT microarray development involving screening for polymorphism and score signature-based redundancy analysis in the prototype and operational arrays (see text for details; n.d. not determined)

Article Snippet: Arrays were spotted using a MicrogridII arrayer (Biorobotics) on poly-L-lysine coated glass microarray slides (Erie Scientific).

Techniques: Microarray, Clone Assay

Journal: Plant Methods

Article Title: A high-density Diversity Arrays Technology (DArT) microarray for genome-wide genotyping in Eucalyptus

doi: 10.1186/1746-4811-6-16

Figure Lengend Snippet: Distribution of the number of polymorphic DArT clones within each binning class in the prototype and interim phases of the DArT microarray development

Article Snippet: Arrays were spotted using a MicrogridII arrayer (Biorobotics) on poly-L-lysine coated glass microarray slides (Erie Scientific).

Techniques: Clone Assay, Microarray

Journal: BMC Biotechnology

Article Title: Protein microarray: sensitive and effective immunodetection for drug residues

doi: 10.1186/1472-6750-10-12

Figure Lengend Snippet: Schematics of the ci-ELISA and protein microarray procedures . 5 μg of the artificial antigens (CL or SM 2 ) are coated onto the 96-well plates in (A) ci-ELISA, or 20 ng of the artificial antigens were spotted onto the protein microarray slides in (B) protein microarray. Antibodies against the particular artificial antigens are added together with a range of concentration of the corresponding hapten. As the haptens would compete with the immobilized artificial antigens for binding to the antisera, an increasing concentration of the hapten would result in a decreasing signal. For ci-ELISA, the bound antibodies are visualized by an anti-rabbit-HRP conjugated antibody with the addition of a chromogen (A). For the protein array, the secondary antibodies are conjugated to a fluorescent dye (Cy3), and the signal can be directly measured by a fluorescence microarray scanner (B).

Article Snippet: Twenty nanogram of each artificial antigen were spotted onto a 7 × 7 array on P-L-L microarray slides (CapitalBio Corporation, Beijing, China) using an OmniGrid-100 Microarrayer (Genomic Solutions, Ann Arbor, MI, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Microarray, Concentration Assay, Binding Assay, Protein Array, Fluorescence

Journal: BMC Biotechnology

Article Title: Protein microarray: sensitive and effective immunodetection for drug residues

doi: 10.1186/1472-6750-10-12

Figure Lengend Snippet: IC 50 of protein microarray immuntodetection and ci-ELISA . Upper panels: Dose-response curves for (A) CL and (B) SM 2 from ci-ELISA and protein microarray. The log of the hapten concentration (x-axis) was plotted against the percentage of inhibition (y-axis), which is (OD sample /OD control ) × 100%. The control group OD was considered to be the point of 100% activity. The IC 50 was determined by non-linear regression analysis. Lower panels: fluorescent signals from the protein microarray slides for (C) CL and (D) SM 2 . The concentration of the standard solution for each slide is: 1: 0 ng/ml; 2: 1 ng/ml; 3: 5 ng/ml; 4: 10 ng/ml; 5: 20 ng/ml; 6: 100 ng/ml; 7: 1000 ng/ml.

Article Snippet: Twenty nanogram of each artificial antigen were spotted onto a 7 × 7 array on P-L-L microarray slides (CapitalBio Corporation, Beijing, China) using an OmniGrid-100 Microarrayer (Genomic Solutions, Ann Arbor, MI, USA).

Techniques: Microarray, Enzyme-linked Immunosorbent Assay, Concentration Assay, Inhibition, Control, Activity Assay

Journal: BMC Biotechnology

Article Title: Protein microarray: sensitive and effective immunodetection for drug residues

doi: 10.1186/1472-6750-10-12

Figure Lengend Snippet: Recoveries of CL from fortified chicken muscle tissues by ci-ELISA and protein microarray

Article Snippet: Twenty nanogram of each artificial antigen were spotted onto a 7 × 7 array on P-L-L microarray slides (CapitalBio Corporation, Beijing, China) using an OmniGrid-100 Microarrayer (Genomic Solutions, Ann Arbor, MI, USA).

Techniques: Microarray

Journal: BMC Biotechnology

Article Title: Protein microarray: sensitive and effective immunodetection for drug residues

doi: 10.1186/1472-6750-10-12

Figure Lengend Snippet: Detection of CL from CL-treated chicken muscle tissues by ci-ELISA and protein microarray

Article Snippet: Twenty nanogram of each artificial antigen were spotted onto a 7 × 7 array on P-L-L microarray slides (CapitalBio Corporation, Beijing, China) using an OmniGrid-100 Microarrayer (Genomic Solutions, Ann Arbor, MI, USA).

Techniques: Microarray